Essex companies Milsoms Hotels and Restaurants, based in Dedham and The Wine Company, based in Colchester, clashed in a charity match at Ipswich Town Football Club.
The hotel group staff triumphed 2 -1 and have so far raised around £30,000 for the Sir Bobby Robson Foundation.
Bosses of the two companies won the chance to play the game, which took place on Sunday, at a charity auction.
Sue Bunting, spokeswoman for Milsom Hotels and Restaurants said: "We still don't know the final amount raised - we are still raising more - but so far we have raised around £30,000 from ticket sales, sale of programmes and the auction itself."
Click here to see a gallery of pictures from the game.